Association of chlorhexidine and high fluoride dentifrice on Streptococcus mutans viability using an in vitro biofilm model

Guilherme Bandeira Santana, Debora Lima Silva, Josie Haydée Lima Ferreira, Fabiana Gouveia Rolim, Glauber Campos Vale


Objective. This in vitro study investigated the effect of Fluoride (F), Chlorhexidine (CHX), and their association on the viability of Streptococcus mutans using a biofilm model. Materials and Methods. Biofilms were anaerobically grown on glass slides that were vertically suspended in 24-well plates for 5 days.  After 48 h of initial growth, biofilms were treated for the next 72 h, 2x/day with CHX at 0.12% and 2%, F as NaF at 0.08% and 0.4% and their association. Results. CHX treatment decreased the bacteria counts either alone or in association with both F concentrations, when compared with control group and the F treatments alone (p<0.05). Conclusion. No addition effect was observed when CHX and F were used in combination, when compared with CHX used alone.     

Full Text: