Evaluation of Salivary and Serum Alpha Amylase Level in Dental Caries of Adolescence

Authors

  • Fatemeh Ahmadi- Motamayel Dental Research Center and Research Center for Molecular Medicine – Department of Oral Medicine – Faculty of Dentistry – Hamadan University of Medical Sciences – Hamadan – Iran.
  • Mohammad Taghi Goodarzi Research Center for Molecular Medicine – Hamadan University of Medical Sciences – Hamadan – Iran.
  • Zohreh Jamshidi Department of Oral Medicine – Faculty of Dentistry – Hamadan University of Medical Sciences – Hamadan – Iran.
  • Ali Mahdavinezhad Medical school – Hamadan University of Medical Sciences – Hamadan – Iran.
  • Nasrin Rafieian Department of Oral Medicine – Faculty of Dentistry – Alborz University Of Medical Sciences – Karaj – Iran.

DOI:

https://doi.org/10.14295/bds.2016.v19i2.1227

Abstract

Objective: Some previous studies suggested a significant relationship between alpha- amylase, and caries formation. This study was implemented in order to investigate the interrelation between level of salivary and serum alpha- amylase and dental caries. Material and Methods: In this cross-sectional investigation, un-stimulated whole saliva  and serum sample was collected from 118 high school students  who were divided to four groups: Caries free female (N= 28), caries active females (N=35), caries free males(N= 28) and caries active males(N= 27). Mean levels of salivary and serum alpha-amylase was assayed by spectrophotometric method to assay enzyme kinetics. Data were analyzed using student’s t-test and chi-square test. Results: The results of this study demonstrated  that salivary and Serum alpha- amylase were  significantly   higher in caries active group as compared to carries free group(P = 0.002, P= 0.001 respectively). In addition in male groups the mean salivary and serum alpha -amylase was significantly higher in caries active as compared to caries free (P = 0.002, P = 0.02 respectively) and in   female Groups the mean serum   alpha- amylase was statistically significant higher in caries active as compared to caries free (P = 0.01). Conclusion: The results of this study demonstrated significant association between salivary and serum alpha- amylase in adolescence with dental caries. More research should be done to demonstrate real relation between   alpha amylase and   dental caries.

Keywords

Alpha–amylase; Dental caries; Saliva; Serum.

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Published

2016-07-07

Issue

Section

Clinical or Laboratorial Research Manuscript