Influence of different hydrogen peroxide neutralizer solutions on the dental root morphology

Abstract

The consequences of the application of antioxidant agents on the root morphology are yet to be fully known. Objective: Evaluate through scanning electron microscopy (SEM) the dental root morphology after the application of different neutralizer solutions, which were submitted to internal tooth bleaching with hydrogen peroxide 35%. Material and Methods: Eighteen bovine incisor teeth were randomly selected and prepared. Then, they were distributed into 6 groups: CG:without bleaching; B:only bleaching; BSA:bleaching + 10% of sodium ascorbate solution for 3 hours; BCH:bleaching + calcium hydroxide paste for 7 days; BA:bleaching + 10% alpha-tocopherol for 3 hours; BC:bleaching + catalase based gel for 2 minutes. The roots were vertically sectioned and analyzed under SEM at 2000x and 5000x magnification. Two photomicrographs, obtained from the cervical third, were randomly selected from each sample using SEM and subsequently evaluated. The images were verified based on a scoring system for smear removal. The Kruskal-Wallis test and Dunn post hoc with Bonferroni correction was performed. Results: It was observed that B presented dentin tubules with larger diameter, BSA presented smaller and partially obliterated tubules, while BCH and BA presented higher formation of precipitates with irregular surface and obliterated tubules. The BC agent showed a cleaner surface, with opening of the tubules similar to the CG group. Groups B, BC and CG were statistically similar. Conclusion: The different neutralizer substances promoted the deposition of precipitate and obliteration of the dentin tubules, being BC the neutralizer substance which generated less interference on the dental root morphology.

KEYWORDS

Antioxidants; Dentin; Hydrogen peroxide; Microscopy; Tooth bleaching.