Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue


  • João Luiz de Miranda Basic Sciences Department – Federal University of Jequitinhonha and Mucuri Valley – Diamantina – MG – Brazil.
  • Dhelfeson Willya Douglas de Oliveira Post-Graduate Program in Dentistry – Federal University of Minas Gerais – Belo Horizonte – MG – Brazil.
  • Rafael Menezes-Silva Graduate Program in Applied Dental Sciences – Bauru Dental School – University of São Paulo – Bauru – SP – Brazil.
  • Roseana de Almeida Freitas Dentistry Department – Federal University of Rio Grande do Norte – Natal – RN – Brazil.



Objective: In this study, the expression of the extracellular matrix proteins was immunohistochemically studied and compared with the histological grading of squamous cell carcinomas of the lower lip and tongue. Material and Methods: The lower lip carcinomas (n=12) and the tongue carcinomas (n=12) were histopathologically graduated according to Bryne’s method. The immunohistochemical technique utilized specific antibodies to collagen IV and laminin. Histopathologic and immunohistochemical analysis were carried-out on the tumoral invasive front. Results: Most of lower lip carcinomas (91.7%) was classified in lower score and all tongue carcinomas (100%) in high score malignant grade (p<0.01). Collagen type IV expression was absent in the peritumoral basement membrane in 50% of lower lip carcinomas and in 66.7% of tongue carcinomas (p=0.09). Laminin expression was absent in the peritumoral basement membrane in 66.7% of lower lip carcinomas and in 58.3% of tongue carcinomas (p=0.48). When these two glicoproteins were expressed, they showed a linear, thin and discontinuous pattern and a weak intensity of expression. Conclusion: The high score malignancy grade of the tongue carcinomas associated with the expression pattern of the studied matrix proteins. It suggests that tongue squamous cell carcinomas have more invasive potential and more aggressive biological behavior than the lower lip carcinomas.



Collagen type IV; Laminin; Carcinoma; Immunohistochemistry.







Clinical or Laboratorial Research Manuscript