Analysis of the volume of autogenous cancellous bone grafts with or without the use of ePTFE membranes in ovariectomized rats.

Authors

  • Maria Aparecida Neves Jardini UNESP - FOSJC
  • Andrea Carvalho De Marco ICT-Faculdade de Odontologia
  • Antônio Braulino de Melo Filho ICT-Faculdade de Odontologia
  • Warley David Kerbauy ICT-Faculdade de Odontologia
  • Rodrigo Dias Nascimento ICT - UNESP - Univ Estadual Paulista
  • Mauro Pedrine Santamaria ICT-Faculdade de Odontologia

DOI:

https://doi.org/10.14295/bds.2013.v16i3.880

Abstract

Objective: To verify the influence of the osteopeny induced by the bone repairing of the receptor site/autogenous bone graft block interface either associated with or without PTFE-e membrane through the analysis of the trabecular bone volume. Material & Methods: 48 Wistar rats weighing approximately 300 g were submitted to parietal bone graft which was fixed to the lateral wall of the left mandibular ramus. The animals were randomly divided into four groups: Group 1: simulated ovariotomy (SHAM) and autogenous bone graft; Group 2: SHAM and autogenous bone graft through PTFE-e membrane recovering; Group 3: ovariotomy (OVZ) and autogenous bone graft; Group 4: OVZ and autogenous bone graft and PTFE-e membrane recovering. The animals of each group were killed at the following periods: 21, 45 and 60 days, comprising 4 animals per group. The pieces were decalcified and included; the cuts were stained with HE and submitted  to histological and histomorphometric analysis through light microscopy. Results: ANOVA test showed that the variables accounting for both the condition (OVZ and SHAM) and the period (21,45 and 60 days) were statistically significant; the Tukey test (5%) showed that the period of 21 days was statistically different from 45 and 60 days; however, they were not statistically different between each other. The descriptive histological analysis showed grafting integration in all animals. Conclusion: the process of graft integration with the receptor site was negatively affected by the induced osteopeny presence and presence or absence of PTFE-e membrane did not interfered in the integration process.

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Published

2013-08-06

Issue

Vol. 16 No. 3 (2013): Jul. - Sep. / 2013 - Published August 2013

Section

Clinical or Laboratorial Research

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