Analysis of the volume of autogenous cancellous bone grafts with or without the use of ePTFE membranes in ovariectomized rats.

Maria Aparecida Neves Jardini, Andrea Carvalho De Marco, Antônio Braulino de Melo Filho, Warley David Kerbauy, Rodrigo Dias Nascimento, Mauro Pedrine Santamaria


Objective: To verify the influence of the osteopeny induced by the bone repairing of the receptor site/autogenous bone graft block interface either associated with or without PTFE-e membrane through the analysis of the trabecular bone volume. Material & Methods: 48 Wistar rats weighing approximately 300 g were submitted to parietal bone graft which was fixed to the lateral wall of the left mandibular ramus. The animals were randomly divided into four groups: Group 1: simulated ovariotomy (SHAM) and autogenous bone graft; Group 2: SHAM and autogenous bone graft through PTFE-e membrane recovering; Group 3: ovariotomy (OVZ) and autogenous bone graft; Group 4: OVZ and autogenous bone graft and PTFE-e membrane recovering. The animals of each group were killed at the following periods: 21, 45 and 60 days, comprising 4 animals per group. The pieces were decalcified and included; the cuts were stained with HE and submitted  to histological and histomorphometric analysis through light microscopy. Results: ANOVA test showed that the variables accounting for both the condition (OVZ and SHAM) and the period (21,45 and 60 days) were statistically significant; the Tukey test (5%) showed that the period of 21 days was statistically different from 45 and 60 days; however, they were not statistically different between each other. The descriptive histological analysis showed grafting integration in all animals. Conclusion: the process of graft integration with the receptor site was negatively affected by the induced osteopeny presence and presence or absence of PTFE-e membrane did not interfered in the integration process.

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