Impact of prenatal protein-calorie malnutrition on the odontogenesis of wistar rats


  • Juliana Schaia Rocha Universidade Estadual de Ponta Grossa
  • Márcia Helena Baldani Department of Dentistry – State University of Ponta Grossa – UEPG – Ponta Grossa – PR – Brazil.
  • Célia Maria da Lozzo Lopes Department de Biology, Genetics and Evolution – State University of Ponta Grossa – UEPG – Ponta Grossa – PR – Brazil.



Objective: This study aimed to determine changes in odontogenesis arising from prenatal and postnatal protein-calorie malnutrition. Materials and methods: Twelve adult Wistar rats were selected; 8 females and 4 males. The females were divided into two groups, one of which received a normoproteic diet (NG) and the other received a hypoproteic diet (HG). After the birth of the litters, 24 pups were randomly separated from each group. The animals were sacrificed, 12 at five-days old and 12 at eight-days old, and their jaws were subjected to histological preparation to obtain cuts of tooth germs. Forty-eight slides were selected that presented the germs properly cut (24 from each group), which were analyzed by microscopy and measured by a calibrated examiner. The differences between means were verified by the nonparametric Mann-Whitney test. Results: The results showed that at 5 days the differences in thickness of enamel and dentin were statistically significant between NG (84.08 ± 28.9 and 141.51 ± 33.2; p = 0.026) and HG (47.26 ± 43.8 and 91.19 ± 54.7; p = 0.006). At 8 days of life there were no significant differences between the groups. Conclusion: The results showed evidence of the impact of malnutrition on the thickness of dental tissues. It is suggested that further work is carried out in this line of research with more complex designs.

Author Biography

Juliana Schaia Rocha, Universidade Estadual de Ponta Grossa

Departamento de Odontologia. Aluna do Programa de Pós Graduação em Odontologia - Área de concentração: Clínica Integrada






Clinical or Laboratorial Research Manuscript