Association of chlorhexidine and high fluoride dentifrice on Streptococcus mutans viability using an in vitro biofilm model

Authors

  • Guilherme Bandeira Santana Dentistry Course of Federal University of Piauí
  • Debora Lima Silva Dentistry Course of Federal University of Piauí
  • Josie Haydée Lima Ferreira Microbiology and Parasitology Department, Federal University of Piauí
  • Fabiana Gouveia Rolim Postgraduate Program in Dentistry, Federal University of Piauí
  • Glauber Campos Vale Restorative Dentistry Department, Federal University of Piauí

DOI:

https://doi.org/10.14295/bds.2016.v19i3.1258

Abstract

Objective. This in vitro study investigated the effect of Fluoride (F), Chlorhexidine (CHX), and their association on the viability of Streptococcus mutans using a biofilm model. Materials and Methods. Biofilms were anaerobically grown on glass slides that were vertically suspended in 24-well plates for 5 days.  After 48 h of initial growth, biofilms were treated for the next 72 h, 2x/day with CHX at 0.12% and 2%, F as NaF at 0.08% and 0.4% and their association. Results. CHX treatment decreased the bacteria counts either alone or in association with both F concentrations, when compared with control group and the F treatments alone (p<0.05). Conclusion. No addition effect was observed when CHX and F were used in combination, when compared with CHX used alone.     

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Published

2016-09-29

Issue

Vol. 19 No. 3 (2016): Jul. - Sep. / 2016 - Published September 2016

Section

Short Communication