Total protein level reduction of odontopathogens biofilms by Rosmarinus officinalis L. (rosemary) extract: an analysis on Candida albicans and Streptococcus mutans

Authors

  • Jonatas Rafael de Oliveira São Paulo State University (Unesp) - Institute of Science and Technology, São José dos Campos - Department of Biosciences and Oral Diagnosis - São José dos Campos – SP - Brazil. http://orcid.org/0000-0003-2398-6506
  • Gabriela de Fátima Santana-Melo São Paulo State University (Unesp) - Institute of Science and Technology, São José dos Campos - Department of Biosciences and Oral Diagnosis - São José dos Campos – SP - Brazil. http://orcid.org/0000-0002-7331-8517
  • Samira Esteves Afonso Camargo University of Florida. College of Dentistry - Department of Restorative Dental Sciences. Gainesville - FL - United States. http://orcid.org/0000-0002-2527-0651
  • Luana Marotta Reis de Vasconcellos São Paulo State University (Unesp) - Institute of Science and Technology, São José dos Campos - Department of Biosciences and Oral Diagnosis - São José dos Campos – SP - Brazil. http://orcid.org/0000-0003-4344-0578
  • Luciane Dias de Oliveira São Paulo State University (Unesp) - Institute of Science and Technology, São José dos Campos - Department of Biosciences and Oral Diagnosis - São José dos Campos – SP - Brazil. http://orcid.org/0000-0002-5465-9551

DOI:

https://doi.org/10.14295/bds.2019.v22i2.1679

Abstract

Objective: The resistance of fungi and bacteria to the available antimicrobials has increased and the development of alternative products to control them has become a very requirement. The use of plant products could be a viable option due to the efficacy, viability, and availability they present. Thereby, this study evaluated the effect of R. officinalis L. extract on C. albicans and S. mutans biofilms, by the total protein level analysis presented by the microorganisms. Material and Methods: For this purpose, monomicrobial biofilms were formed for 48 h and exposed to the R. officinalis L. extract for 5 min. Then, total protein quantification was performed by Lowry method. Results: The analysis showed significant total protein reduction of the biofilms after exposure to the plant extract, with 39 ± 11%, for C. albicans, and 32 ± 11%, for S. mutans. Conclusion: R. officinalis L. extract decreased the total protein level in both biofilms. Thus, C. albicans and S. mutans protein composition could be a target for action of antimicrobial agents.

Keywords

Biofilms; Candida albicans; Proteins; Rosmarinus officinalis; Streptococcus mutans.

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Published

2019-04-30

Issue

Vol. 22 No. 2 (2019): Apr. - Jun. / 2019 - Published April 2019

Section

Clinical or Laboratorial Research