Expression of BMP II by human osteoblasts cultivated on dense or porous titanium

Authors

  • Carolina Fedel Gagliardi São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Luana Marotta Reis de Vasconcellos São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Carlos Alberto Alves Cairo Division of Materials – Air and Space Institute – São José dos Campos – SP – Brazil.
  • Sylvia Bicalho Rabelo São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Restorative Dentistry – SP – Brazil.
  • Renata Falchete do Prado São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2018.v21i3.1586

Abstract

Objective: To quantified the expression of the morphogenetic bone protein II (BMP II) produced by human cells with osteoblast differentiation, after cultured over dense or porous samples of pure titanium grade II. Material and methods: The samples were produced by powder metallurgy technique. Mesenquimal steam cells isolated from alveolar bone of healthy donors were stimulated to differentiate, assuming an osteoblastic phenotype, by supplemented medium and plated over the samples. The experimental groups were: control, dense titanium, titanium of higher porosity and titanium of lower porosity. After 7 and 14 days, the RNA was extracted. The cDNA was prepared and the polymerase chain reaction (PCR) technique was used in real time, using the constitutive gene Beta-Actin to relative quantification. The pore morphology and interconnection were confirmed by Scanning Electron Microscopy (SEM). Results: Total porosity (obtained after apply dimensions and density formulas) and  surface porosity (SEM) presented significant differences among the groups. For the group of total porosity of 33.79%, the superficial porosity was 32.5% (± 7.74%) and for the group of 41.79%, the superficial porosity was 37.4% (± 7.95%), significantly lower. The expression of BMP II was similar in all groups. Conclusion: It was concluded that the powder metallurgy has a reduced ability to standardize the porosity in the samples and that the porosity does not interfere in the cellular response of BMP II production, an important inducer of osteoblastic differentiation.

 

Keywords

Porous titanium; BMP II; Cell culture; PCR.

Downloads

Published

2018-08-01

Issue

Vol. 21 No. 3 (2018): Jul. - Sep. / 2018 - Published Aug 2018

Section

Clinical or Laboratorial Research

License

COPYRIGHT TRANSFER AND RESPONSIBILITY STATEMENT
 (PDF | Word)

All manuscript's copyright ownership of the article "____________________________________________________________________" is transferred from the author(s) to the BRAZILIAN DENTAL SCIENCE, in the event the work is published. The manuscript has not been published elsewhere and that it has not been submitted simultaneously for publication elsewhere.

We hereby attest the study is original and does not present manipulated data, fraud or plagiarism. We made significant scientific contribution to the study and we are aware of the presented data and in agreement with the final version of the manuscript. We assume complete responsibility for the ethical aspects of the study.

 

This text must be printed and signed by all authors. The scanned version should be submitted as supplemental file during the submission process.