Different conditioning agents provide TGF-β1 release from root dentin: an in vitro study

Abstract

Objective: This study evaluated the effectiveness of different conditioning agents in promoting the release of Transforming Growth Factor Beta 1 (TGF-β1) from root dentin. Material and Methods: Sixty-eight third molars were distributed into four groups (n=17): 17% ethylenediaminetetraacetic acid (EDTA); 10% EDTA; 10% citric acid (CA); CA + 3% ferric chloride (10-3 solution). Slices of the cervical third of the root were obtained and irrigated with 2% chlorhexidine for 5 min. The slices were immersed in 220 μL of each conditioning agent for 10 min to expose TGF-β1 and analyzed by ELISA. Data normality was tested using the Shapiro-Wilk test. Kruskal-Wallis tests with Dunn-Bonferroni post hoc were used for comparison between groups (p<0.05). Results: Significant differences were observed among the treatments (p<0.001). The lowest TGF-β1 release was found in the group treated with 17% EDTA, which was significantly lower compared to the 10% EDTA, 10% CA and 10-3 solution groups. Conclusion: It could be concluded that different conditioning agents significantly affect the release of TGF-β1 from root dentin. Among the tested agents, 17% EDTA resulted in the lowest release, whereas 10% EDTA and the 10–3 solution promoted higher levels of growth factor release.

KEYWORDS

Dentin; Endodontics; Growth factor beta 1; Regeneration; Regenerative endodontics.