Evaluating Cytotoxic Effects of Highly Esthetic Dental Composites

Numan Aydın, Serpil Karaoğlanoğlu, Elif Aybala Oktay, Aysun Kılıç Süloğlu

Abstract


Amaç: Diş hekimliği alanında nanoteknoloji alanında odaklı diş kompozitler, ön ve arka dişlerin olduğu yerlerde yaygın olarak yapılır. L929 fare fibroblast sitotoksik olduğu için in vitro ekstrakt test yöntemi ile araştırıldı.

Gereç ve Yöntemler: Steril bir dolapta cam moddaki led ışık cihazıyla polimerleştirilerek 8x2 mm çapında kompozit numuneler hazırlandı. Kompozit numuneler yüzey plakaları (3 cm ISO standartlarına göre 2 / ml hesaplandı ), 37 ° C sıcaklıkta, 24 ve 72 saat süre ile inkübe edildi O C. Hücre canlılığı, 3- [4,5-dimetiltiyazol-2-il ile değerlendirildi] -2,5-difeniltetrazolyum bromür (MTT) deneyi ve hücre ölümü laktat dehidrojenaz (LDH) sızıntı deneyi ile değerlendirildi.

Sonuçlar: 24 saatin sonunda kompozitlerin 1: 1 ekstraktları (nanoseramik kompozit hariç) toksik etki göstermedi. Kompozit numunelerin 1: 1 ekstrelerinin hücre canlılığı sonuçları, 72 saat sonra analiz edileceği halde, kontrol grubuna göre anlamlı farklılıklar bulundu (p <0.05).

Sonuç: Dolgu tipinin ve boyutunun kompozitlerin toksisitesinde etkili olması ve organik matrikslerde Bis-GMA, TEGDMA, UDMA ve Bis EMA monomerlerini içeren kompozitlerin istenildiği yerde canlı canlılığı (% 70) gösterdiği görülmektedir. ISO tarafından söyildi. Orada birlikte, organik matrislerinde PEGDMA ve BPA monomerlerine sahip olan, sahip olan kompozitler,% 70'in olduğu kabul edilebilir seviyesinin altında olan ve toksik olan bir ve Türkiye'nin sahip ve zayıf ortamdaki canlılığı vardır.


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DOI: https://doi.org/10.14295/bds.2020.v23i1.1883