Cytotoxic Effects of Bulk-Fill Composites on L929 Fibroblast Cells

Authors

  • Numan Aydın University of Health Sciences, Gulhane Faculty of Dentistry, Department of Restorative Dental Treatment https://orcid.org/0000-0001-8628-4507
  • Serpil Karaoğlanoğlu University of Health Sciences, Gulhane Faculty of Dentistry, Department of Restorative Dental Treatment
  • Elif Aybala Oktay University of Health Sciences, Gulhane Faculty of Dentistry, Department of Restorative Dental Treatment
  • Aysun Kılıç Süloğlu Hacettepe University, Faculty of Science Department of Biology, Ankara, Turkey

DOI:

https://doi.org/10.14295/bds.2021.v24i1.2146

Abstract

Objective: Unlike traditional composite resins, bulk-fill composite resins could be polymerized as thicker layers. This study aims to contribute to the field by investigating the cytotoxic effects of various bulk-fill composite resins on L929 mouse fibroblast cells in vitro. Material and Methods: In our study, six bulk fill and one conventional composite resin were used. Composite resin samples (8×4 mm) were prepared in a sterile cabinet by using a glass mod and polymerizing with a led light device (DTE LUX E, Germany). Composite samples (n:3) of which surface area was calculated according to ISO 10993-12: 2012 standards (3 cm2/ml), were kept in media for 24 h and 72 h in 37 oC incubator, their extracts were filtered in 1:1 and 1:2 proportion and were added on L929 mouse fibroblast cells. Cell viability was examined by the MTT assay and cell death by the LDH test. Cell viability results were evaluated using one-way analysis of variance (ANOVA) test (p<0.05). Results: When the 1:1 extracts from 4 mm thick bulk-fill composite samples were applied on L929 mouse fibroblast cells, cell viability rates showed significant differences compared to the control group at the end of 24 h and 72 h (except for Estelite Bulk Fill Flow). Although the extracts of the tested composite samples at 1:1 and 1:2 ratio at the end of 72 hours caused a decrease in L929 mouse fibroblast cell viability, the cell viability rate of only PRG-containing bulk fill composite and conventional composite remained below the cell viability ratio (70%) specified in ISO standards. Bulk fill composites did not produce toxic effects (except Beautifil Bulk Restorative) according to the LDH test. Conclusions: Despite decreasing in general the cell viability, bulk-fill composite resins used in 4 mm thick layers provided cell viability rates over the acceptability level, except PRG-containing bulk fill composite (Beautifil Bulk Restorative), which was cytotoxic to L929 mouse fibroblasts.

Keywords

Bulk fill composite; Cytotoxicity; L929 cells; LDH assay.

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Published

2020-12-22 — Updated on 2021-12-21

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Issue

Vol. 24 No. 1 (2021): Jan - Mar / 2021 - published Dec 2020

Section

Clinical or Laboratorial Research

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All manuscript's copyright ownership of the article "____________________________________________________________________" is transferred from the author(s) to the BRAZILIAN DENTAL SCIENCE, in the event the work is published. The manuscript has not been published elsewhere and that it has not been submitted simultaneously for publication elsewhere.

We hereby attest the study is original and does not present manipulated data, fraud or plagiarism. We made significant scientific contribution to the study and we are aware of the presented data and in agreement with the final version of the manuscript. We assume complete responsibility for the ethical aspects of the study.

 

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