Characterization of sound enamel and natural white spot lesions
DOI:
https://doi.org/10.4322/bds.2022.e3488Abstract
Objective: To compare optical, morphological, chemical, and physical aspects of the sound enamel and white
spot lesions (WSL) classified as ICDAS 2. Material and Methods: Seventeen human molars with one surface
presenting WSL and a sound surface (2 x 2 mm window) were characterized by Quantitative light-induced
fluorescence (QLF®), Optical coherence tomography (OCT), microhardness, and Raman spectroscopy. The ANOVA
and Tukey’s test were used at 5% significance level. Results: The QLF comparison between distinct substrates
yielded decreased Q (integrated fluorescence loss) of -15,37%mm2 and -11,68% F (fluorescence loss) for
WSL. The OCT detected mean lesion depth of 174,43 um. ANOVA could not detect differences in the optical
attenuation coefficient between the substrates (p>0.05). Lower microhardness measures were observed in WSL
than on sound enamel (p<0.05). The Raman spectra showed four vibrational phosphate bands (v1, v2, v3, v4),
where the highest peak was at 960.3 cm-1 (v1) for both substrates. However, a 40% decrease in phosphate (v1)
was detected in WSL. The peak at 1071 cm-1 was higher for sound enamel, indicating the presence of a phosphate
band instead of the B-type carbonate. The spectra showed higher intensity of the organic composition at 1295 cm-1
and 1450 cm-1 for WSL. Conclusion: Non-invasive QLF, OCT and Raman spectroscopy were able to distinguish
differences in fluorescence, optical properties, and organic/inorganic components, respectively, between sound
enamel and WSL, validated by the destructive microhardness analysis.
KEYWORDS
Dental caries; Dental enamel; White-spot lesions; Diagnosis; Raman Spectroscopy.
Downloads
Published
Issue
Section
License
COPYRIGHT TRANSFER AND RESPONSIBILITY STATEMENT
(PDF | Word)
All manuscript's copyright ownership of the article "____________________________________________________________________" is transferred from the author(s) to the BRAZILIAN DENTAL SCIENCE, in the event the work is published. The manuscript has not been published elsewhere and that it has not been submitted simultaneously for publication elsewhere.
We hereby attest the study is original and does not present manipulated data, fraud or plagiarism. We made significant scientific contribution to the study and we are aware of the presented data and in agreement with the final version of the manuscript. We assume complete responsibility for the ethical aspects of the study.
This text must be printed and signed by all authors. The scanned version should be submitted as supplemental file during the submission process.