Cytotoxicity and antibacterial activity of bleached turmeric hydro-alcoholic extract versus sodium hypochlorite as an endodontic irrigant: in vitro study
DOI:
https://doi.org/10.4322/bds.2022.e3566Abstract
Objective: To evaluate the cytotoxicity and antibacterial effect of bleached turmeric hydro-alcoholic extract
compared to sodium hypochlorite as an endodontic irrigant. Material and Methods: Cytotoxicity was evaluated
on human skin fibroblasts using MTT assay. The irrigants were tested at 1, 5- and 15-minutes time intervals. After
contact time, MTT solution was added and well plates were incubated. After the incubation period, optical density
was read and correlated with cell viability percent. Antibacterial efficiency was evaluated using direct contact test.
Each endodontic irrigant was added to fresh Enterococcus faecalis suspension and brain heart infusion media then
incubated for 48 hours. After incubation period, optical density readings were obtained and read by ELISA reader
at 620 nm. Results: Results of cytotoxicity test revealed that bleached turmeric extract had significant higher cell
viability percent than Sodium hypochlorite (NaOCl) at all time intervals (p<0.001). However, in the intervention
group, cell viability percent significantly decreased over time. Results of antibacterial test showed bacterial inhibition
by both groups with non-significant difference between the two tested groups (p<0.05). Conclusion: Bleached
turmeric hydro-alcoholic extract can represent an herbal alternative endodontic irrigant to avoid the undesirable
toxic effects of NaOCl due to its less cytotoxicity and prominent antibacterial effect against Enterococcus faecalis.
KEYWORDS
Antibacterial effect; Bleached turmeric; Cytotoxicity; Endodontic irrigantion; Sodium hypochlorite.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Brazilian Dental Science uses the Creative Commons (CC-BY 4.0) license, thus preserving the integrity of articles in an open access environment. The journal allows the author to retain publishing rights without restrictions.
=================
COPYRIGHT TRANSFER AND RESPONSIBILITY STATEMENT
(PDF)
For all articles published in the BDS journal, copyright is retained by the authors. Articles are licensed under an open-access Creative Commons CC BY 4.0 license, meaning that anyone may download and read the paper for free. In addition, the article may be reused and quoted, provided that the original published version is cited. These conditions allow for maximum use and exposure of the work while ensuring that the authors receive proper credit. All metadata associated with published articles is released under the Creative Commons CC0 Universal Public Domain Dedication.
Before the submission, authors must obtain permission to reproduce any published material (figures, schemes, tables, or any extract of a text) that does not fall into the public domain or for which they do not hold the copyright. Permission should be requested by the authors from the copyright holder (usually the Publisher, please refer to the imprint of the individual publications to identify the copyright holder).
The authors hereby attest that the study is original and does not present manipulated data, fraud, or plagiarism. All names listed made a significant scientific contribution to the study, are aware of the presented data, and agree with the final version of the manuscript. They assume complete responsibility for the ethical aspects of the study.
This text must be printed and signed by all authors. The scanned version should be submitted as supplemental file during the submission process.
