Resin Nano-Ceramic Material Surface Roughness, Color Stability, and Bacterial Adhesion Evaluation After Different Scaling Methods




Objectives: The study evaluated in vitro the changes in roughness, color stability, and bacterial count of a CAD/CAM Resin Nano-Ceramic material surface treated by various scaling procedures. Material and Methods: 70 disks (5mm diameter, 0.5 mm thickness) of Resin Nano-Ceramic (Lava ™Ultimate, 3M, ESPE) material were cemented in standardized cavities prepared in bovine teeth. A custom-made scaling apparatus of a double pan balance was used for different scaling methods, simulating standard clinical conditions. The specimens were assigned to three main groups: no scaling(C), ultrasonic scaling (U), and manual scaling (M). Each group was then divided into three subgroups according to scaling tip material; stainless steel tip (St), plastic tip (P), and titanium tip (Ti). The surface texture was analyzed quantitatively and qualitatively with a tactile profilometer and atomic force microscopy. A spectrophotometer was used for color measurement. Streptococcus mutans were counted in a colony counter. All the data were tabulated and statistically analyzed. Results: Two-way ANOVA was used to study the effect while One-way ANOVA was performed to compare between study groups. The significance level was set at p 0.05. The ultrasonic titanium tip(UTi) revealed the significant highest mean value of alterations (p < 0.001). The integrity of the material surface was altered in the form of deep scratches on the ultrasonically scaled surfaces and numerous smaller scratches on the hand-scaled surfaces. Conclusion: The plastic instrument would appear to be the instrument of choice during a routine maintenance procedure for Resin Nano-Ceramic materials.


Bacterial biofilm; Dental ceramics; Color stability; Scaling; Surface roughness.

Author Biographies

Engy Adel Ahmed Farag, The British University in Egypt, Fixed Prosthodontics,Faculty of Dentistry,Cairo,Egypt

Lecturer in fixed Prosthodontics Department in the faculty of dentistry at The British University in Egypt

Tarek Salah Morsi , Ain Shams University,Fixed Prosthodontics, Faculty of Dentistry, Cairo,Egypt.

Professor of fixed prosthodontics ,faculty of dentistry,Ain Shams  University

Marwa Mohamed Wahsh, Ain Shams University,Fixed Prosthodontics, Faculty of Dentistry, Cairo,Egypt

Associate professor of fixed prosthodontics,faculty of dentistry,Ain Shams University

Amr Saleh El-Etreby, Ain Shams University,Fixed Prosthodontics, Faculty of Dentistry, Cairo,Egypt

Associate professor of fixed prosthodontics,Ain Shams University,Faculty of Dentistry, Cairo,Egypt






Clinical or Laboratorial Research Manuscript