Efeito do anticorpo monoclonal 56G sobre o crescimento de Streptococcus mutans em caldo e no acúmulo de placa bacteriana in vitro

Authors

  • Antonio Carlos Victor Canettieri Faculdade das Ciências da Saúde – UNIVAP
  • Fujiko Y. Kretchetoff Faculdade de Odontologia de São José dos Campos
  • Fabio C. Fujarra Faculdade de Medicina – USP
  • Daniella Moreira Faculdade de Odontologia de São José dos Campos – UNESP
  • Carmelinda S. Unterkircher Faculdade de Odontologia de São José dos Campos – UNESP

DOI:

https://doi.org/10.14295/bds.2006.v9i4.461

Abstract

O anticorpo monoclonal (Acmo) anti-Streptococcus mutans 56G foi analisado quanto ao efeito na aderência e crescimento da placa bacteriana in vitro, e quanto ao crescimento em caldo e no comprimento da cadeia do estreptococo. O Acmo foi purifi cado em proteína A - Sepharose e testado em ELISA frente a quatro cepas de S. mutans (ATCC 35668, SP, GS5, CD28A). O Acmo reconheceu com maior intensidade a cepa GS5. As placas bacterianas formadas in vitro na presença do Acmo apresentou menos carboidrato total (polissacarídeo extracelular) que o grupo controle e essa diferença foi signifi cante (p>0,001). A placa bacteriana do grupo tratado, formada sobre uma lamínula de vidro, era diferente da placa controle e apresentava agregados menores que cobriam a lamínula quase completamente. O Acmo 56G não infl uenciou o crescimento bacteriano, nem o comprimento da cadeia de S. mutans.Os resultados desse trabalho sugerem que o Acmo testado deve reconhecer a glicosiltransferase (GTF) de S. mutans presente na superfície bacteriana, interferindo na síntese de glucano por essa enzima.

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Published

2010-08-16

How to Cite

1.
Canettieri ACV, Kretchetoff FY, Fujarra FC, Moreira D, Unterkircher CS. Efeito do anticorpo monoclonal 56G sobre o crescimento de Streptococcus mutans em caldo e no acúmulo de placa bacteriana in vitro. BDS [Internet]. 2010 Aug. 16 [cited 2025 Aug. 25];9(4). Available from: https://bds.ict.unesp.br/index.php/cob/article/view/461

Issue

Vol. 9 No. 4 (2006)

Section

Clinical or Laboratorial Research

License

Brazilian Dental Science uses the Creative Commons (CC-BY 4.0) license, thus preserving the integrity of articles in an open access environment. The journal allows the author to retain publishing rights without restrictions.

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