The incorporation of chlorhexidine in dental prosthetic materials may interfere on its microbial colonization and surface roughness: a preliminary study

Abstract

Objective: the present study aimed to evaluate in vitro the antimicrobial activity and roughness interference of relining materials of removable acrylic resin-based prosthesis whenever incorporated with 1% chlorhexidine diacetate. Material and Methods: For that purpose, New TrulinerTM and TrusoftTM relining resins probes were made with and without the incorporation of the compound. The antimicrobial activity against Candida albicans ATCC 10231 of the probes was evaluated by the agar diffusion test, using the inhibition halo measurement and by colony forming units counting. Quantitative analyses of both relining materials roughness were obtained in a non-contact 3D digital profilometer. Results: Both materials incorporated with chlorhexidine exhibited reasonable inhibition halos demonstrating the ability to inhibit C. albicans growth, in a time-dependent manner. Although an increase in the roughness of both materials was observed over time (p<0.05), the incorporation of chlorhexidine did not alter the surface roughness significantly (p>0.05). Conclusion: Considering the above, this study suggests that the relining materials incorporated with 1% chlorhexidine diacetate have an important antimicrobial activity against C. albicans, with no considerable changes in surface roughness and may be considered a suitable tool against prosthetic stomatitis.

KEYWORDS

Antimicrobial activity; Candida albicans; Chlorhexidine; Prosthetic stomatitis; Relining material.