Microbiological evaluation of dental stone casts after immersion in sodium hypochlorite and peracetic acid

Autores

  • Livia Lie Sonoda Sekiguchi São Leopoldo Mandic Institute and Dental Research Center – Campinas – SP – Brazil.
  • Ricardo Takiy Sekiguchi Dental School and University Nove de Julho – São Paulo – SP – Brazil.
  • Milton Edson Miranda São Leopoldo Mandic Institute and Dental Research Center http://orcid.org/0000-0002-5345-5776
  • Roberta Tarkany Basting São Leopoldo Mandic Institute and Dental Research Center – Campinas – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2016.v19i1.1242

Resumo

Objective: the purpose of this study was to evaluate the efficacy of disinfection of type III dental stone by immersion in 1% sodium hypochlorite and 0.25% peracetic acid at different periods of time (1, 5 and 10 min). MaterialandMethods: silicon dies were previously infected with strains of Bacillus subtilis for 15 min. Then, type III gypsum stone (Herodent, Vigodent COLTÈNE SA, Rio de Janeiro, Brazil) was inserted into the cavities to obtain contaminated specimens. A sterile silicone die was used to obtain uncontaminated specimens. The specimens were separated into positive and negative control groups, and further divided into the following groups: blocks immersed in sterile physiologic solution for 1, 5 or 10 min; blocks immersed in 1% sodium hypochlorite for 1, 5 or 10 min; and blocks immersed in 0.25% peracetic acid for 1, 5 or 10 min. All the groups were double-plated and incubated at 37 ?C for 24 h. Results: the results were expressed in colony forming units (CFU/ml) and the data were submitted to the Kruskal-Wallis test followed by Dunn’s test. The results showed that immersion in 1% sodium hypochlorite and 0.25% peracetic acid resulted in complete disinfection of the test specimens at all test periods (p < 0.01), whereas immersion in saline did not provide effective disinfection. Conclusion: it can be concluded that both 1% sodium hypochlorite and 0.25% peracetic acid provided effective disinfection in dental stone specimens immersed in the solutions described above, at different periods of time.

Keywords

Disinfection; Peracetic acid; Sodium hypochlorite; Stone casts.

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Biografia do Autor

Milton Edson Miranda, São Leopoldo Mandic Institute and Dental Research Center

DDS, MSc, PhD, professor, Department of Restorative Dentistry

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Publicado

2016-03-14

Como Citar

1.
Sekiguchi LLS, Sekiguchi RT, Miranda ME, Basting RT. Microbiological evaluation of dental stone casts after immersion in sodium hypochlorite and peracetic acid. BDS [Internet]. 14º de março de 2016 [citado 19º de setembro de 2025];19(1):106-12. Disponível em: https://bds.ict.unesp.br/index.php/cob/article/view/1242

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Artigos de Pesquisa Clínica ou Laboratorial

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