Alternative therapies for denture stomatitis treatment: in vivo experimental model in rats

Guilherme Rodrigues Teodoro, Gabriela de Morais Gouvêa Lima, Marina Bacelar Silva, Patricia Carla Lopes, Cybelle Mori Hiraoka, Gabriela de Fátima Santana-Melo, Daniel Freitas Alves Pereira, Fernanda Lourenção Brighenti, Cristiane Yumi Koga-Ito


Background: Denture stomatitis (DS) is a multifactorial condition that commonly affects denture users and is mainly caused by Candida albicans. Due to the toxic effects of antifungal therapy, new therapies for DS are claimed. Objective: The aim of the study was to evaluate the efficacy of aqueous extract of Buchenavia tomentosa and sodium bicarbonate against C. albicans in a model of DS in rats. Material and Methods: An acrylic resin device simulating a denture base was fixed covering the palate of forty-eight male rats followed by candidiasis induction. Rats were divided into 4 groups (n=12): Control, sodium bicarbonate, B. tomentosa and nystatin (positive control). Each group was subdivided according to the period of treatment; 24 hours (n=6) and 48 hours (n=6). Animals were sacrificed and had their devices removed for C. albicans counts and SEM analysis. The palate mucosa was removed and processed for histopathologic analysis. Results: After 24 hours of treatment, both B. tomentosa and nystatin groups reduced significantly C. albicans counts when compared to control (nystatin x control, p<0.01; B. tomentosa x control, p=0.03). The results were confirmed by the histologic analysis. Conclusion: Both the aqueous extract of B. tomentosa and sodium bicarbonate was able to significantly decrease C. albicans counts in an experimental model of DS.

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