Antimicrobial and mechanical acrylic resin properties with silver particles obtained from Fusarium oxysporum

Authors

  • Tabata Prado Sato São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Dental Materials and Prosthodontics – SP – Brazil.
  • Celio Ilidio Conjo Higher Institute of Health Sciences (ISCISA), Maputo, Mozambique.
  • Rodnei Dennis Rossoni São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Juliana Campos Junqueira São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Biosciences and Oral Diagnosis – SP – Brazil.
  • Renata Marques de Melo São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Dental Materials and Prosthodontics – SP – Brazil.
  • Nelson Durán Institute of Chemistry – University of Campinas – Campinas – SP – Brazil.
  • Alexandre Luiz Souto Borges São Paulo State University (Unesp) – Institute of Science and Technology – São José dos Campos – Department of Dental Materials and Prosthodontics – SP – Brazil.

DOI:

https://doi.org/10.14295/bds.2018.v21i1.1534

Abstract

Objective: This study evaluated the effects of the incorporation of silver nanoparticles (AgNPs) obtained from Fusarium oxysporum on heat-activated acrylic resin (HAAR) and their influence on resin’s surface roughness, hardness, color alteration and antimicrobial capacity against Candida albicans. Material and Methods: For this, 50 discs of HAAR (2x5 mm) were produced and divided into three groups, Control: HAAR; Ag1: HAAR plus 0.539 mg of AgNPs; and Ag2: HAAR plus 1.1 mg of AgNPs. Knopp hardness (HK), surface roughness (Ra and Rz) and color alteration according to the CIE Lab were measured. Specimens were then evaluated in vitro with regard to C. albicans biofilm formation through formed colony count (CFU/mL). Scanning Electron Microscopy (SEM) and Atomic force microscopy (AFM) analyses were performed. Results: The addition of AgNPs of both concentrations changed Ra, Rz and HK significantly. There was statistically significant difference for L (p=0.00); a*(p=0.00) and b*(p=0.00) parameters. There were no differences between Ag1 and Ag2 biofilm formation, but the comparison of both with the control group presented a significant reduction (p=0.0091) on biofilm formation. SEM and AFM images showed no signs of NPs clustering. Conclusion: It can be concluded tha AgNPs incorporation in HAAR was effective in reducing C. albicans activity, with a slight change in color and hardness of the material, being effective therefore, in regions such as the dental prostheses palate, which have lesser aesthetic appeal.

Keywords

Acrylic resins; Silver; Antimicrobial agents.

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Published

2018-03-28

Issue

Vol. 21 No. 1 (2018): Jan. - Mar. / 2018 - Published March 2018

Section

Clinical or Laboratorial Research