Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest

Authors

  • Jonatas Rafae de Oliveira Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Univ Estadual Paulista/UNESP, São José dos Campos, SP, Brazil http://orcid.org/0000-0003-2398-6506
  • Daiane de Jesus São Paulo State University (UNESP)
  • Luciane Dias de Oliveira Universidade Estadual paulista (UNESP)

DOI:

https://doi.org/10.14295/bds.2017.v20i1.1317

Abstract

Objective: This study evaluated the effect of rosemary extract on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa monomicrobial biofilms viability, as well as on C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms. Material and Methods: In microtiter plate, mono- and polymicrobial biofilms for 48 h were formed. Then, they were exposed for 5 min to rosemary extract (200 mg/mL). Saline (0.9% NaCl) was used as control. After, washes were done with saline to remove the affected cells. Biofilm viability was checked by MTT colorimetric assay, after treatment. Absorbance of the wells was read in microplate spectrophotometer (570 nm) and data were converted to reduction percentage and statistically analyzed by ANOVA and Tukey test (p ? 0.05). Results: After application of rosemary extract, with exception of the E. faecalis biofilm, significant reductions in mono- and polymicrobial biofilms viability were observed. Conclusion: C. albicans, S. aureus, S. mutans and P. aeruginosa monomicrobial biofilms were affected by rosemary extract, as well as C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms, presenting significant viability reductions.   

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Published

2017-02-20

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Section

Clinical or Laboratorial Research Manuscript