909 - Development of Candida albicans biofilm in acrylic resin submitted to treatment with plant extracts

Resumo

Objective: To evaluate the antifungal potential of Equisetum arvense L. (horsetail), Glycyrrhiza glabra L. (licorice), Punica granatum L. (pomegranate) and Stryphnodendron barbatimam Mart. (barbatimão) extracts, after Candida albicans biofilm formation on acrylic resin. Material and methods: C. albicans standard strain was cultured on Sabouraud-dextrose agar for 24 h at 37°C. After standardized in a spectrophotometer, 100 µL of the inoculum (10⁶ cells/mL) and a sterile acrylic resin disc were maintained in Brain Heart Infusion broth supplemented with sucrose (5%), for 5 days at 37ºC. The samples of the treated groups (n = 10) were separately exposed to a concentration of 50 mg/mL of each extract for 5 minutes or to nystatin (48.83 IU/mL). For the untreated group (control, n = 10) was used sterile saline (0.9% NaCl). Biofilms were disaggregated of the acrylic resin discs by an ultrasonic homogenizer for 30 s. After decimal dilutions, sowings in Sabouraud-dextrose plates were made with incubation for 48 h at 37°C. Later, CFU/mL was counted and the values were converted to log₁₀ and had their statistical analysis done (ANOVA and Tukey Test, p ? 0.05).  Results: It was found that all plant extracts and nystatin resulted in significant C. albicans biofilm reduction (p < 0.01) compared to the control group (0.9% NaCl). However, all of them showed similar reductions to each other (p = 0.1567). Conclusion: There was biofilm formation of C. albicans on acrylic resin and all plant extracts were effective against this yeast, acting similarly to nystatin.